hACE2(ECD), Avi/His-Tag

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trenzyme SKU: P2020-170_100

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Tag: Avi/His-Tag C-terminal not-biotinylated
Variant: A25V K26E K31N E35K N64I L79F N90H

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Description

The human Angiotensin-Converting Enzyme 2 (ACE-2) is a protein highly expressed at the surface of cells of the human lung, arteries, kidney, heart and intestine and is shown to be the entry receptor for SARS-CoV-2 infection. The ACE-2 protein is one of the most important components for Coronavirus research to develop effective therapies for COVID-19. Engineered ACE2 mutants carrying specific mutations, as specified in the following, are characterized by highly increased binding affinities to the receptor-binding protein (RBD) of SARS-CoV-2 spike protein.

P2020-170, P2020-182: A25V, K26E, K31N, E35K, N64I, L79F, N90H 
P2020-171, P2020-183: A25V, K31N, E35K, L79F 
P2020-172, P2020-184: K31M, E35K, Q60R, L79F 
P2020-173, P2020-185: T20I, H34A, T92Q, Q101H

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Overview
 Sequence Information Product Information Background Information Download
  • Product Name: hACE2(ECD), Avi/His-Tag
  • Catalog No.: P2020-170, P2020-171, P2020-172, P2020-173, P2020-182, P2020-183, P2020-184, P2020-185
  • RefSeq Links: UniProt: Q9BYF1
  • Synonyms: hACE2; ACEH; human angiotensin-converting enzyme 2; ACE-related carboxypeptidase; Angiotensin-converting enzyme homolog; Metalloprotease MPROT15; biotinylated hACE2; non-biotinylated hACE2
  • Species: Homo sapiens
  • Tags: Avi/His-Tag, C-terminal, Avi-Tag not biotinylated; Avi/His-Tag, C-terminal, Avi-Tag biotinylated
  • Sequence without tags for P2020-170: (AA 20-707):

    MTIEEQVETFLDNFNHKAEDLFYQSSLASWNYNTNITEENVQNMNIAGDKWSAFLKEQST
    FAQMYPLQEIQHLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNP
    QECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYED
    YGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISP
    IGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSV
    GLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGH
    IQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINF
    LLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYC
    DPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNML
    RLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSI
    KVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPR
    ISFNFFVTAPKNVSDIIPRTEVEKAIRMS
  • Sequence without tags for P2020-171: (AA 20-707):

    MTIEEQVKTFLDNFNHKAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQST
    FAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNP
    QECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYED
    YGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISP
    IGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSV
    GLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGH
    IQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINF
    LLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYC
    DPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNML
    RLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSI
    KVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPR
    ISFNFFVTAPKNVSDIIPRTEVEKAIRMS
  • Sequence without tags for P2020-172: (AA 20-707):

    MTIEEQAKTFLDMFNHKAEDLFYQSSLASWNYNTNITEENVRNMNNAGDKWSAFLKEQST
    FAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNP
    QECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYED
    YGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISP
    IGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSV
    GLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGH
    IQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINF
    LLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYC
    DPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNML
    RLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSI
    KVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPR
    ISFNFFVTAPKNVSDIIPRTEVEKAIRMS
  • Sequence without tags for P2020-173: (AA 20-707):

    MIIEEQAKTFLDKFNAEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQST
    LAQMYPLQEIQNLQVKLQLQALHQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNP
    QECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYED
    YGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISP
    IGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSV
    GLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGH
    IQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINF
    LLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYC
    DPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNML
    RLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSI
    KVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPR
    ISFNFFVTAPKNVSDIIPRTEVEKAIRMS
  • Sequence without tags for P2020-182: (AA 20-707): MTIEEQVETFLDNFNHKAEDLFYQSSLASWNYNTNITEENVQNMNIAGDKWSAFLKEQSTFAQMYPLQEIQHLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMS
  • Sequence without tags for P2020-183: (AA 20-707): MTIEEQVKTFLDNFNHKAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQST
    FAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNP
    QECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYED
    YGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISP
    IGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSV
    GLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGH
    IQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINF
    LLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYC
    DPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNML
    RLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSI
    KVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPR
    ISFNFFVTAPKNVSDIIPRTEVEKAIRMS
  • Sequence without tags for P2020-184: (AA 20-707): MTIEEQAKTFLDMFNHKAEDLFYQSSLASWNYNTNITEENVRNMNNAGDKWSAFLKEQST
    FAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNP
    QECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYED
    YGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISP
    IGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSV
    GLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGH
    IQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINF
    LLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYC
    DPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNML
    RLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSI
    KVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPR
    ISFNFFVTAPKNVSDIIPRTEVEKAIRMS
  • Sequence without tags for P2020-185: (AA 20-707): MIIEEQAKTFLDKFNAEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLQVKLQLQALHQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMS
  • Please note:
    Methionine at pos. 1 might be present due to cloning constraints.
    C-terminal Avi-tag and His-tag not shown in sequence.
    X indicates mutation sites.
  • Expression Host: HEK293
  • Formulation: PBS; pH 7.4
  • Format: Liquid, stored and shipped at -80° C
  • Purity: > 90% as determined by SDS-PAGE; > 95 % as determined by SDS-PAGE
  • Application: ELISA, Functional Assay

The human Angiotensin-Converting Enzyme 2 (hACE-2) is a type I transmembrane metallocarboxypeptidase with homology to ACE, a regulator in the Renin-Angiotensin system (RAS) and long-known as a target for the treatment of hypertension.

hACE2 is expressed at the surface of cells of the human lungs, arteries, kidneys, heart and intestine – all tissues shown to harbor SARS-CoV. The function of ACE-2 is known as controlling blood pressure. This is accomplished by the hydrolysis of a small peptide hormone called Angiotensin II into the nonapeptide Angiotensin 1-9, which is thereafter converted into the heptapeptide angiotensin 1-7 by ACE and other endopeptidases. Angiotensin 1-7 acts in a vasoconstricting manner and is believed to be one of the main effectors in controlling the blood pressure and is therefore involved in pathophysiological processes like diabetes, hypertension and cardiac function in general. Recently it became known, that the new Coronavirus SARS-CoV-2 uses ACE-2 as the entry point into alveolar cells of the lungs, where it replicates and causes the Coronavirus disease (COVID-19).​


Additional information for P2020-170: hACE2(ECD) (A25V, K26E, K31N, E35K, N64I, L79F, N90H), Avi/His-Tag

SDS-PAGE/Coll. Coomassie

Histogram of marked lane in gel picture
SDS Page for hACE2 ECD A25V K26E K31N E35K N64I L79F N90H Avi His-Tag
Histogram for hACE2 ECD A25V K26E K31N E35K N64I L79F N90H Avi His-Tag

Additional information for P2020-171: hACE2(ECD) (A25V, K31N, E35K, L79F), Avi/His-Tag

SDS-PAGE/Coll. Coomassie

Histogram of marked lane in gel picture
SDS Page for hACE2 ECD A25V K31N E35K L79F Avi His-Tag
Histogram for hACE2 ECD A25V K31N E35K L79F Avi His-Tag

Additional information for P2020-172: hACE2(ECD) (K31M, E35K, Q60R, L79F), Avi/His-Tag

SDS-PAGE/Coll. Coomassie

Histogram of marked lane in gel picture
SDS Page for hACE2 ECD K31M E35K Q60R L79F Avi His-Tag
Histogram for hACE2 ECD K31M E35K Q60R L79F Avi His-Tag

Additional information for P2020-173: hACE2(ECD) (T20I, H34A, T92Q, Q101H), Avi/His-Tag

SDS-PAGE/Coll. Coomassie

Histogram of marked lane in gel picture
SDS Page for hACE2 ECD T20I H34A T92Q Q101H Avi His-Tag
Histogram for hACE2 ECD T20I H34A T92Q Q101H Avi His-Tag

 

Additional information for P2020-182: Biotinylated hACE2(ECD) (A25V, K26E, K31N, E35K, N64I, L79F, N90H), Avi/His-Tag

SDS-PAGE/Coll. Coomassie

Histogram of marked lane in gel picture
SDS-Page for Biotinylated hACE2(ECD) (A25V, K26E, K31N, E35K, N64I, L79F, N90H), Avi/His-Tag
Histogram for Biotinylated hACE2(ECD) (A25V, K26E, K31N, E35K, N64I, L79F, N90H), Avi/His-Tag

Additional information for P2020-183: Biotinylated hACE2(ECD) (A25V, K31N, E35K, L79F), Avi/His-Tag

SDS-PAGE/Coll. Coomassie

Histogram of marked lane in gel picture
SDS-Page for Biotinylated hACE2(ECD) (A25V, K31N, E35K, L79F), Avi/His-Tag
Histogram for Biotinylated hACE2(ECD) (A25V, K31N, E35K, L79F), Avi/His-Tag

Additional information for P2020-184: Biotinylated hACE2(ECD) (K31M, E35K, Q60R, L79F), Avi/His-Tag

SDS-PAGE/Coll. Coomassie

Histogram of marked lane in gel picture
SDS-Page for Biotinylated hACE2(ECD) (K31M, E35K, Q60R, L79F), Avi/His-Tag
Histogram for Biotinylated hACE2(ECD) (K31M, E35K, Q60R, L79F), Avi/His-Tag

Additional information for P2020-185: Biotinylated hACE2(ECD) (T20I, H34A, T92Q, Q101H), Avi/His-Tag

SDS-PAGE/Coll. Coomassie

Histogram of marked lane in gel picture
SDS-Page for Biotinylated hACE2(ECD) (T20I, H34A, T92Q, Q101H), Avi/His-Tag
Histogram for Biotinylated hACE2(ECD) (T20I, H34A, T92Q, Q101H), Avi/His-Tag

Download Product Information for P2020-170: hACE2(ECD) (A25V, K26E, K31N, E35K, N64I, L79F, N90H), Avi/His-Tag

Download Product Information for P2020-171: hACE2(ECD) (A25V, K31N, E35K, L79F), Avi/His-Tag

Download Product Information for P2020-172: hACE2(ECD) (K31M, E35K, Q60R, L79F), Avi/His-Tag

Download Product Information for P2020-173: hACE2(ECD) (T20I, H34A, T92Q, Q101H), Avi/His-Tag

Download Product Information for P2020-182: Biotinylated hACE2(ECD) (A25V, K26E, K31N, E35K, N64I, L79F, N90H), Avi/His-Tag

Download Product Information for P2020-183: Biotinylated hACE2(ECD) (A25V, K31N, E35K, L79F), Avi/His-Tag

Download Product Information for P2020-184: Biotinylated hACE2(ECD) (K31M, E35K, Q60R, L79F), Avi/His-Tag

Download Product Information for P2020-185: Biotinylated hACE2(ECD) (T20I, H34A, T92Q, Q101H), Avi/His-Tag

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