hACE2(ECD), Avi/His-Tag

• Product Name: hACE2(ECD), Avi/His-Tag
  
• Catalog No.: P2020-170, P2020-171, P2020-172, P2020-173
  
• RefSeq Links: UniProt: Q9BYF1
  
• Synonyms: hACE2; ACEH; human angiotensin-converting enzyme 2; ACE-related carboxypeptidase; Angiotensin-converting enzyme homolog; Metalloprotease MPROT15; non-biotinylated hACE2

• Species: Homo sapiens
• Tags: Avi/His-Tag, C-terminal, Avi-Tag not biotinylated
• Sequence without tags for P2020-170: (AA 20-707):
  
  MTIEEQVETFLDNFNHKAEDLFYQSSLASWNYNTNITEENVQNMNIAGDKWSAFLKEQST
  FAQMYPLQEIQHLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNP
  QECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYED
  YGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISP
  IGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSV
  GLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGH
  IQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINF
  LLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYC
  DPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNML
  RLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSI
  KVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPR
  ISFNFFVTAPKNVSDIIPRTEVEKAIRMS
  
• Sequence without tags for P2020-171: (AA 20-707):
  
  MTIEEQVKTFLDNFNHKAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQST
  FAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNP
  QECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYED
  YGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISP
  IGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSV
  GLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGH
  IQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINF
  LLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYC
  DPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNML
  RLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSI
  KVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPR
  ISFNFFVTAPKNVSDIIPRTEVEKAIRMS
  
• Sequence without tags for P2020-172: (AA 20-707):
  
  MTIEEQAKTFLDMFNHKAEDLFYQSSLASWNYNTNITEENVRNMNNAGDKWSAFLKEQST
  FAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNP
  QECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYED
  YGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISP
  IGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSV
  GLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGH
  IQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINF
  LLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYC
  DPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNML
  RLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSI
  KVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPR
  ISFNFFVTAPKNVSDIIPRTEVEKAIRMS
  
• Sequence without tags for P2020-173: (AA 20-707):
  
  MIIEEQAKTFLDKFNAEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQST
  LAQMYPLQEIQNLQVKLQLQALHQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNP
  QECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYED
  YGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISP
  IGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSV
  GLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGH
  IQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINF
  LLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYC
  DPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNML
  RLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSI
  KVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPR
  ISFNFFVTAPKNVSDIIPRTEVEKAIRMS
• Please note:
  Methionine at pos. 1 might be present due to cloning constraints.
  C-terminal Avi-tag and His-tag not shown in sequence.
  X indicates mutation sites.

• Expression Host: HEK293
  
• Formulation: PBS; pH 7.4
  
• Format: Liquid, stored and shipped at -80° C
• Purity: > 90% as determined by SDS-PAGE
  
• Application: ELISA, Functional Assay

The human Angiotensin-Converting Enzyme 2 (hACE-2) is a type I transmembrane metallocarboxypeptidase with homology to ACE, a regulator in the Renin-Angiotensin system (RAS) and long-known as a target for the treatment of hypertension.

hACE2 is expressed at the surface of cells of the human lungs, arteries, kidneys, heart and intestine – all tissues shown to harbor SARS-CoV. The function of ACE-2 is known as controlling blood pressure. This is accomplished by the hydrolysis of a small peptide hormone called Angiotensin II into the nonapeptide Angiotensin 1-9, which is thereafter converted into the heptapeptide angiotensin 1-7 by ACE and other endopeptidases. Angiotensin 1-7 acts in a vasoconstricting manner and is believed to be one of the main effectors in controlling the blood pressure and is therefore involved in pathophysiological processes like diabetes, hypertension and cardiac function in general. Recently it became known, that the new Coronavirus SARS-CoV-2 uses ACE-2 as the entry point into alveolar cells of the lungs, where it replicates and causes the Coronavirus disease (COVID-19).​

Additional information for P2020-170: hACE2(ECD) (A25V, K26E, K31N, E35K, N64I, L79F, N90H), Avi/His-Tag

SDS-PAGE/Coll. Coomassie	Histogram of marked lane in gel picture

Additional information for P2020-171: hACE2(ECD) (A25V, K31N, E35K, L79F), Avi/His-Tag

SDS-PAGE/Coll. Coomassie	Histogram of marked lane in gel picture

Additional information for P2020-172: hACE2(ECD) (K31M, E35K, Q60R, L79F), Avi/His-Tag

SDS-PAGE/Coll. Coomassie	Histogram of marked lane in gel picture

Additional information for P2020-173: hACE2(ECD) (T20I, H34A, T92Q, Q101H), Avi/His-Tag

SDS-PAGE/Coll. Coomassie	Histogram of marked lane in gel picture

Download Product Information for P2020-170: hACE2(ECD) (A25V, K26E, K31N, E35K, N64I, L79F, N90H), Avi/His-Tag

Download Product Information for P2020-171: hACE2(ECD) (A25V, K31N, E35K, L79F), Avi/His-Tag

Download Product Information for P2020-172: hACE2(ECD) (K31M, E35K, Q60R, L79F), Avi/His-Tag

Download Product Information for P2020-173: hACE2(ECD) (T20I, H34A, T92Q, Q101H), Avi/His-Tag