Our hiMicroglia provide a robust, physiologically relevant in vitro model for studying their diverse functions. Generated from human iPSC using a optimized and highly standardized differentiation protocol, the cells closely resemble native human microglia by expressing the key marker IBA1, while exhibiting essential functional characteristics such as cytokine release and inflammatory responsiveness.
The cryopreserved cells are fully differentiated and ready to use for your assay from Day 1. The cells recover efficiently after thawing (viability 85-90%), enabling consistent experimental performance while reducing hands-on time and batch-to-batch variability.
In addition to monoculture studies, these hiMicroglia are ideally suited for co-culture CNS models to investigate neuroimmune crosstalk, synaptic remodeling and disease-relevant cellular interactions.
trenzyme has developed a production process for microglia from iPSC cells (human iPSC-derived). Please note: Microglia for research and development applications (RUO). Not for human or animal clinical trials, therapeutic or diagnostic use.
Additional information for hiMicroglia, assay-ready
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Fig. 1: Immunofluorescence staining of IBA1 (red) in hiMicroglia on day 1 after thawing. Nuclei are stained by Hoechst33342 (blue). A) Scale bar: 50 µm B) Scale bar: 20 µm
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Fig. 2: hiMicroglia morphology upon thawing from D1 to D7. Scale bar: 100 µm
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Fig. 3: NF-κB translocation assay in hiMicroglia on day 1 after thawing. Cells were stimulated with 1 µg/ml LPS for 1 hour followed by 4% PFA fixation and anti-NF-kB p65 staining. A) Calculation of the NF-κB fluorescence intensity ratio (nucleus/cytoplasm) to quantify the LPS-induced (1 µg/ml) NF-κB translocation from cytoplasm into the nucleus in comparison to ctrl (untreated) cells. B) Exemplary immunofluorescence stainings of iMicroglia for NF-κB assay. NF-κB p65 (green) in the cytoplasm of ctrl (untreated) cells and predominantely in the nucleus of cells treated with 1 µg/ml LPS. Nuclei are stained by Hoechst33342 (blue). Scale bar: 20 µm
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Fig. 4: LPS-induced IL6 secretion in hiMicroglia. On day 1 after thawing, cells were stimulated with 1 µg/ml LPS for 48 hours, then cell culture supernatants were harvested and IL6 secretion was analysed via ELISA. n.d = not detected.
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