Cleavage and Tag Control Protein


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SKU: P2020-141 trenzyme

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The Cleavage and Tag Control Protein is a versatile tool consisting of various elements and cleavage sites for different proteases. Furthermore, it's equipped with multiple tags to serve as positive control for Western Blot analyses or functional assays.

The Cleavage and Tag Control Protein is available in liquid or lyophilized form. The format and size can be selected by using the buttons.


  • Product Name: Cleavage and Tag Control Protein
  • Catalog No.: P2020-131 liquid, P2020-141 lyophilized
  • Synonyms: control protein, reference protein, cleavage control protein, multiple tag control protein, universal reference protein
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Customer Testimonial

“We highly valued the fast and excellent communication and the high flexibility of the team! For any future project, we will preferably entrust in trenzyme’s protein production services.”

Dr. Thore Hettmann
Probiodrug AG, Halle/Saale, Germany

Sequence Information

  • Species: artificial
  • Tags: Twin-Strep, HA, T7, Avi, FLAG, and His-tag; N-terminal MBP and C-terminal sfGFP fusion

Product Information

  • Expression Host: E. coli
  • Formulation: PBS; pH 7.4
  • Format:
    Liquid: stored and shipped at -80 °C.
    Lyophilized: stored at -80 °C and shipped at ambient temperature. We recommend reconstituting the protein in ddH2O. In case of 10 µg, add 11 µl ddH2O.
  • Purity: > 99 % (measured by densitometry of Coomassie stained gel, see below)

Background Information

The Cleavage and Tag Control Protein is a versatile protein consisting of various cleavage sites for proteases including TEV, Enterokinase, Thrombin, Factor Xa and PreCission. Protease cleavage results in generation of two or three fragments that have significantly reduced molecular weights, which are as follows:
- TEV cleavage: 42.8 kDa and 47.7 kDa
- Enterokinase: 43.5 kDa, 20.1 kDa and 26.9 kDa
- Thrombin: 43.9 kDa and 46.6 kDa
- Factor Xa: 44.3 kDa and 46.2 kDa
- PreCission: 45.3 kDa and 45.3 kDa
Moreover, the 90.5 kDa fusion protein is equipped with multiple tags such as Twin-Strep-, HA-, FLAG-, and His-tag, for instance, that may serve as positive control performing specific Western Blot analyses or any functional assay. Since the protein is fused to GFP, it is additionally applicable as fluorescence control. Thus, the Cleavage and Tag Control Protein provides an excellent tool to monitor protease cleavage reactions and specific Western Blot analyses as well as fluorescence experiments.


SDS-PAGE/Coll. Coomassie

Histogram of marked lane in gel picture

SDS-PAGE of Test Cleavage
Histogram (of marked lane in gel picture) of Test Cleavage

Test Cleavage

Description of Test Cleavage

SDS-PAGE of Test Cleavage
Description of Test Cleavage

Test Cleavage

Description for Test Cleavage

SDS-PAGE of Test Cleavage
Description of Test Cleavage

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