human IL-6, His-tag


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SKU: P2020-140 trenzyme

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The pro-inflammatory cytokine interleukin-6 (IL-6) plays a crucial role in acute inflammatory responses. In response to infection or tissue injury, IL-6 stimulates the production of acute phase reactants, hematopoiesis as well as the differentiation of naïve CD4+ T lymphocytes into IL-17 producing helper T cells. Interestingly, IL-6 is also described as anti-inflammatory myokine, a cytokine, which is produced from muscle cells during exercises. Dysregulation of IL-6 expression is associated with chronic inflammation and autoimmunity. Blockade of IL-6 mediated signaling cascades by using monoclonal antibodies is a promising and effective therapeutic approach to treat a variety of immune-mediated diseases. Moreover, IL-6 has been proposed as biomarker for the development of severe acute respiratory syndrome coronavirus 2, since secretion of IL-6 is significantly increased in COVID-19 patients and associated with poor prognosis due to extensive lung injury.


  • Product Name: human Interleukin-6, His-tag
  • Catalog No.: P2020-140
  • RefSeq Links: HGNC:6018; NX_P05231; NP_000591.1; NP_000591.1NM_000600.4; PDBe 1il6 A; UniProt: P05231
  • Synonyms: Interleukin-6, IL-6, IL6, B-cell stimulatory factor 2 (BSF-2), BSF2, CTL differentiation factor (CDF), Hybridoma growth factor, Interferon beta-2 (IFN-beta-2), IFNB2
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Sequence Information

  • Species: Homo sapiens
  • Tags: His-tag, C-terminal
  • Sequence without tags (AA 30-212):

Product Information

  • Expression Host: HEK293
  • Formulation: PBS; pH 7.4.
  • Format: Liquid, stored and shipped at -80 °C
  • Purity: > 85% as determined by SDS-PAGE
  • Application: ELISA, WB, Functional assay

Background Information

Interleukin-6 (IL-6) is an important pro-inflammatory cytokine, which belongs to the type I cytokine family and is produced by a variety of cell types, including mononuclear phagocytes, dendritic cells, vascular endothelial cells and fibroblasts. Signaling is induced by binding of IL-6 to the cytokine-binding polypeptide chain IL-6R alpha, leading to association of IL-6R alpha with the signal-transducing subunit gp130, together constituting the IL-6 receptor. Expression of the classical IL-6 receptor is restricted to hepatocytes, monocytes, some resting lymphocytes and some epithelial cells. Remarkably, alternative splicing and proteolytic cleavage generate soluble forms of IL-6R alpha allowing IL-6 trans-signaling by binding of IL-6/IL-6R alpha complexes to gp130-expressing cells. Thereby, the range of IL-6 responsive cells is markedly increased as gp130 is ubiquitously expressed.

Both pathogen-associated molecular patterns (PAMPs) and damage-associated molecular patterns (DAMPs) induce the secretion of IL-6, which has both local and systemic effects. Next to IL-6, tumor necrosis factor (TNF)-alpha and IL-1 beta are produced, which promote further synthesis of IL-6.

IL-6 is a crucial mediator of the acute phase response and fever. It stimulates the synthesis of acute phase reactants by hepatocytes, such as the C-reactive protein (CRP), serum amyloid A (SAA) and fibrinogen, and inhibits production of albumin, transferrin and fibronectin. Furthermore, IL-6 secretion leads to increased production of neutrophils in the bone marrow.

The differentiation of naïve CD4+ T cells into IL-17 producing helper T cells is stimulated by IL-6, whereas differentiation into regulatory T cells is concomitantly inhibited. Additionally, IL-6 promotes differentiation of activated B cells into antibody-producing plasma cells, all of which contributes to acquired immunity.

However, dysregulation of IL-6 production causes several chronic inflammatory diseases and autoimmunity. Overproduction of IL-6 has been detected in the synovial cells of rheumatoid arthritis, swollen lymph nodes of the Castleman’s disease, myeloma cells, and peripheral blood cells. With tocilizumab, a humanized monoclonal antibody, an effective therapeutic drug targeting IL-6 signaling cascades has been developed to treat immune-mediated diseases.

In contrast to its pro-inflammatory properties, IL-6 also acts as an anti-inflammatory myokine, which is a cytokine produced by contracting muscle cells.

SDS-PAGE/Coll. Coomassie

Histogram of marked lane in gel picture

P2020-140 human IL-6 interleukin sds-page P2020-140 human IL-6 interleukin histogram

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