Proteinase K, liquid formulation


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SKU: P2020-125 trenzyme

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Proteinase K is a broad-spectrum serine protease originally isolated from the fungus Engyodontium album (formerly Tritirachinm album).


  • Product Name: Proteinase K, liquid formulation
  • Catalog No.: P2020-125
  • RefSeqLinks: UniProt: P06873
  • Synonyms: Endopeptidase K, Tritirachium alkaline proteinase
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Sequence Information

  • Species: Tritirachium album
  • Tags: Tag-free
  • Sequence (AA 106-384):
    No DNase contamination detected by digestion of λ DNA at 37 ℃ for 6 hours. No RNases contamination detected by digestion of λ DNA at 25℃ for 16 hours. Host genomic DNA/RNA is no residual detected by PCR.

Product Information

  • Expression Host: Pichia pastoris
  • Formulation: Supplied as a 0.22 μm filtered solution in 20mM Tris-Hcl, 30mM NaCl, 50% Glycerol, pH 7.2-7.4
  • Format: Liquid, stored and shipped at -80°C
  • Purity: > 95% as determined by SDS-PAGE

Background Information

The recombinant enzyme is a mutant to the native protease, which gains higher specific activity and yield as well as wider pH and temperature range . The large scale recombinant preparation has advantage in lot-to-lot consistency, superior purity and cost-efficiency. DNA-free nature of recombinant Proteinase K made it well-suited in isolating PCR and RT-PCR templates.
Crystal structure data indicate that the enzyme belongs to the subtilisin family, which is characterized by a catalytic triad (Asp39-His69-Ser224) in the active site.
Proteinase K does not exhibit pronounced cleavage specificity, and the preferred cleavage site is the peptide bond at hydrophobic amino acids.

DNase Residual Testing - at 37°C for 12h

Stability testing - Proteinase K solution at 37°C for 360 days

DNase Residual Testing Graph - Proteinase K
Stability testing Proteinase K

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