Furin, GFP/His-tag

• Product Name: Furin, GFP/His-Tag
  
• Catalog No.: P2020-119
• RefSeq Links: UniProt: P09958; NP_001276752.1; NM_001289823.1; NP_001276753.1; NM_001289824.1; NP_002560.1; NM_002569.3; PIR: A39552; GeneID: 5045
• Synonyms: Furin (Paired Basic Amino Acid Cleaving Enzyme); PCSK3; PACE; FUR; SPC1; Paired Basic Amino Acid Residue-Cleaving Enzyme; EC 3.4.21.75; Paired Basic Amino Acid Cleaving Enzyme (Furin, Membrane Associated Receptor Protein); Proprotein Convertase Subtilisin/Kexin; EC 3.4.21

• Species: Homo sapiens, human
• Tags: GFP/His-Tag, C-terminal
• Sequence without tags (AA 27-715):
  MQKVFTNTWAVRIPGGPAVANSVARKHGFLNLGQIFGDYYHFWHRGVTKRSLSPHRPRHS
  RLQREPQVQWLEQQVAKRRTKRDVYQEPTDPKFPQQWYLSGVTQRDLNVKAAWAQGYTGH
  GIVVSILDDGIEKNHPDLAGNYDPGASFDVNDQDPDPQPRYTQMNDNRHGTRCAGEVAAV
  ANNGVCGVGVAYNARIGGVRMLDGEVTDAVEARSLGLNPNHIHIYSASWGPEDDGKTVDG
  PARLAEEAFFRGVSQGRGGLGSIFVWASGNGGREHDSCNCDGYTNSIYTLSISSATQFGN
  VPWYSEACSSTLATTYSSGNQNEKQIVTTDLRQKCTESHTGTSASAPLAAGIIALTLEAN
  KNLTWRDMQHLVVQTSKPAHLNANDWATNGVGRKVSHSYGYGLLDAGAMVALAQNWTTVA
  PQRKCIIDILTEPKDIGKRLEVRKTVTACLGEPNHITRLEHAQARLTLSYNRRGDLAIHL
  VSPMGTRSTLLAARPHDYSADGFNDWAFMTTHSWDEDPSGEWVLEIENTSEANNYGTLTK
  FTLVLYGTAPEGLPVPPESSGCKTLTSSQACVVCEEGFSLHQKSCVQHCPPGFAPQVLDT
  HYSTENDVETIRASVCAPCHASCATCQGPALTDCLSCPSHASLDPVEQTCSRQSQSSRES
  PPQQQPPRLPPEVEAGQRLRAGLLPSHLPE
  
  

• Expression Host: human, HEK293
• Formulation: PBS; pH 7.4.
• Format: Liquid, stored and shipped at -80°C
• Purity: > 85% as determined by SDS-PAGE

Furin, also known as paired basic Amino acid Cleaving Enzyme (PACE) is a ubiquitous subtilisin-like proprotein convertase with a minimal cleavage site of Arg-X-X-Arg˅. However, the enzyme prefers the site Arg-X-Lys/Arg-Arg˅.
It is the major processing enzyme of the secretory pathway and is localized in the trans-golgi network where it functions to cleave other proteins into their mature/active forms.
Substrates of Furin include blood clotting factors, serum proteins and growth factor receptors such as the insulin-like growth factor receptor but also viral proteins like the SARS-CoV-2 Spike protein.
Furin is inhibited by EGTA, α1- Antitrypsin Portland and polyarginine compounds.

SDS-PAGE/Coll. Coomassie	Histogram of marked lane in gel picture

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